endstream endobj startxref NOTE: The BD Accuri CFlow Importer reads the FCS data directly from the BD Accuri CFlow file, so you do not have to export individual files. BD Accuri C6 flow cytometer for the analysis of plant genome sizes. In addition, C6 has a flow metering system for automatic calculation of absolute cell counts. BD Accuri C6 Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1. 21. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. ?���[��? A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. Install, update, and run anti-virus protection software on a regular basis. Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. Such wear includes scratches, scrapes, and general dirtiness. ... Deionised water is recommended in the C6 manual - try this and it may stop blockages. Operate the instrument as described in this Instruction Manual. Peristaltic Pumps and Luer Locks, Figure 4-5. Installation Dialog Box .....80 Figure C-3. x���M��� `(-X�:��&�̑� r�p�N��S�F?�R4�S�^L=�� I��t�_�n@Q4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_4%_`���=��@J�'� �������(�⍴?�aV(�S��'3*{�����f����߫�!cE��+MH4~������U1Qy+��R�L�Vj��\i@���|����f�M�ZY?�`��bJ�ܠD��Ke��@��T`�e���8�HQ�W�8�7�y����R�T���p�]����ޣ�y.� nW�$�����[�}�k�[j�-J�����[�C�+�gA-�G鳅������g��6v���, ����R�s%�Yu���f�y'��8l���N%. The instrument will begin a 5 minute auto start cycle. endstream endobj 2779 0 obj <>stream Flow cytometry within reach The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. Sliding Luer Lock Fittings Off of Pump Head, Figure 5-2. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. Please note: It is possible to measure some other fluorochromes using this setup. Accuri C6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to FACSCalibur. BD Accuri C6 Flow Cytometer Instrument Manual Bd Accuri C6 Pdf User Manuals. Came with note that it needs repair. 661085). The BD Accuri C6 Plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the single-cell level. The software for Accuri C6 (CFlow Plus) is very user-friendly and efficient. Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual . %PDF-1.6 %���� Accuri C6 flow cytometer fits in any lab and is readily accessible to all researchers—experts and novices alike. BD Accuri C6: BMSB J538. Additional laser configurations (2-blue 2-red, 4-blue) are available with the Selectable Lasers Module (Cat. BD Accuri C6 Instrument Manual (31 pages), Figure 1-1. the BD Accuri C6 Flow Cytometer System, BD Accuri C6 Flow Cytometer Specifications, Figure 3-1. You are viewing an Accuri C6 Flow Cytometer. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. endstream endobj 2780 0 obj <>stream Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). View online or download Bd Accuri C6 Instrument Manual Bd Accuri C6 Manuals | ManualsLib BD Accuri™ C6 Flow Cytometer Optical Filter Guide . �! BD Accuri C6 Instrument Manual (31 pages) Flow Cytometer. BD Accuri™ C6 Flow Cytometer Instruction Manual, serial number 2400 and higher; BD Accuri™ C6 Software User Guide; BD CSampler™ Accessory Installation Guide; BD CSampler™ Software User Guide Accuri™ C6 Plus BD Biosciences. ���r��n�Ypm8I_�À�W�"v����,,{�m��c__$�`�r݀��\NiC�{i\ �p=�ƫ�k.x���8�ႃ3��{a�> �6111111111111111��Ǧ�� If the BD Accuri software displays the message Extra Start-up time needed due to cleaning or improper shut down it will take the instrument several more minutes for the instrument to get to the green-light ready state. BD Accuri™ C6 Compensation Calculator (Microsoft® Excel®) BD Accuri™ C6 Flow Cytometer Optical Filter Guide . BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. ۟��@��7����O����vƁ`���OW�p�>����8\:�7k������������>q=���!�\�$/-dzCL��)��"�nÒ��2fVo.C��I�aj���~��`��-`v�T�͖(V�` �㆔����rHf ��d)R�7��V�� ר�Gˌ%b��j�Z��.�f>�Z�i-h�`*G-�mA�S,�1&;e,��R�h�Q�4 Lh&4��{�,a��T���.hݓ�t�D�4,�L��J�'�uI�(Y�22��(�t�O�+L�op���z�¢exy����w�c#s����{�u^�(�����H� In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. For Research Use Only. Since the C6 fluidics system is non-pressurized, any brand of sample tube that is 12 x 75 mm or smaller can be used. Compare the new data to that provided with the C6 and email the file created in step 20 to TechSupport@AccuriCytometers.com *Steps may be skipped if you purchased your computer from Accuri with your C6. Standard fluorochrome in use are identical to FACS Calibur. Core Size/Flow Rate Combinations.....79 Figure C-1. Removing Peristaltic Pump Retainer Clip, Figure 4-6. With minor signs of normal wear and tear. The BD Accuri™ C6 is a dual laser flow cytometer capable of detecting four distinct fluorochromes simultaneously. Includes all accessories shown in pictures. BD Accuri C6 Software (CFlow Plus) controls the BD Accuri C6 flow cytometer system in order to acquire data, generate statistics, and analyze results. 3. h�bbd```b`` � ��9������"ɸD�NB��!�6�[��f����3>��������L�\�`��X��?�� �YF Even more colors to choose from with selectable lasers and filter replacement. Laboratory Equipment BD CSampler Installation Manual 2 pages. Services available on the BD Accuri: Single or multi-color cellular analysis (phenotyping, intracellular staining, etc.) Accuri C6 is a digital PC based bench top analyser, 2 laser system. Update Driver Software - Cytometer, Second Dialog Box, Figure 5-4. !� �XB4��u*��yo7�5�(8.����.�����jo���U߅Ţ?=_��Jk X�3^��=8l�S����6ؽ�6]�w����Wߵ %��S&!bM\w��d�T�����&�B��~��s˟1U�R�l1V&�f�PL�L��K6�c 6�9� The BD Accuri™ C6 Plus personal flow cytometer is the newest generation of the BD Accuri™ platform. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. h�b```d``ja`c``�� Ā B,@Q�G����^�M�*O�:.:�c�a��"~��!������Q���ȵ�۵8�1}p�l�����C�+x��L��a:_"�����.��r+��枘�! So the choice should be addressed by the kind of flow experiments you plan to do. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. Click here to view the C6 software user guide. User Tracking Installer Icon .....80 Figure C-2. Accuri C6 consumables, manuals, etc. It also includes … Such counts are essential in many research and clinical applications, including enumerating leucocytes, B cells, T cells, and platelets in human blood, measuring of … �e�I�c~w1���0 ��� Click here to view the BD Fluorescence Spectraviewer.. No. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. Available to trained users with online booking. DNA/Cell cycle analysis BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express ™ software and FlowJo ™ software. User's Guides and Manuals. Related Manuals for BD Accuri C6 . Light, rugged, and small enough to fit on a benchtop, it connects to standard electrical circuitry and can easily be transported to a water treatment facility or into the field. Along with traditional forward and side scatter, the C6 is an ideal instrument for basic analytical cytometry. �R'�B$K1�#��L�f����2Φ� 5gM�(�,��ycT�}�b��yL]��:y�y�:��~�Kw�s������b\Pע��._H�vtt哂n�mO*���n�s����;;�5����~��;;a��t��~�HX�ÛK x���MkA�����dI#��b�!�@K =�BH}JK���z,�;��i��w����+���n����s�ج�b;���H The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. The unique fluidics system of the BD Accuri C6 Plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. BD Accuri C6 Flow Cytometer Optics and Fluidics (Top View, Lid Open), Changing the Workstation Computer Settings for Windows ® 7, Figure 3-5. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. Overall very good cosmetic condition. The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic … If you are interested in using a fluorochrome that is not listed here with the Accuri C6, please contact the operator.. Click here to view the instrument manual. The BD Accuri® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. 2798 0 obj <>/Filter/FlateDecode/ID[<3B235E56AD4D884590D8BFA25210CF82><756487A21B96FC4983BB2B9C4996548C>]/Index[2774 31]/Info 2773 0 R/Length 99/Prev 1549724/Root 2775 0 R/Size 2805/Type/XRef/W[1 3 1]>>stream This manual provides information on cytometer requirements and specifications, setup, and maintenance. endstream endobj 2775 0 obj <>/Metadata 476 0 R/Pages 2772 0 R/StructTreeRoot 613 0 R/Type/Catalog>> endobj 2776 0 obj <>/MediaBox[0 0 612 792]/Parent 2772 0 R/Resources<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 2777 0 obj <>stream The Accuri C6 cytometer in my lab has some problems, and we need some help. Figure 1-1. endstream endobj 2781 0 obj <>stream Works with current protocols and reagents: Accuri developed the C6 to use the same reagents you currently use on the market-leading cytometers. This includes microfuge tubes and tubes made of polystyrene and polypropylene. y`�B}�@h�K�v�f�)�*Z�6D�&d'��f�z-C�Zn����R73�nǦ3�-m\�v��tN��ƫ�Zij7��um�zc�&��݌�d7�.� ��L�q����5�H=�Y�pQAں΃��^̑�]#�26m�H-���2'd��5g�Z1GZ;��i�� 9kK:::�;+:8��;:��+::X�;*�,v ����ţ����A(� This item has been powered on, but no further testing. Press the Power button on the BD Accuri C6 instrument face panel. 2804 0 obj <>stream A unique, non-pressurized, peristaltic pump system drives the fluidics. Many thanks, Matt . Select the BD Accuri CFlow file (.c6) that contains the data you want to view with FCS Express. The BD Accuri® C6 personal flow cytometer can simplify cell analysis by counting absolute cell numbers directly (per unit sample volume). %%EOF ��qq��p�{|�wtxs endstream endobj 2778 0 obj <>stream 0 `� T���4 d*u�y�Dn�cK�u��A�aZC���_��q�� �#��� Proper Attachment of Fluidics Lines, Figure 4-1. Accuri C6 consumables, manuals, etc. Item is sold AS IS. 2774 0 obj <> endobj For a more complete set of directions refer to the BD Accuri C6 Flow Cytometer Instrument Manual . It also includes common troubleshooting help and service information. Automated Preventive Maintenance Notification Window, Figure 4-4. BD Accuri C6 Plus fluorochrome support All fluorochromes in the table can be detected by the BD Accuri C6 Plus equipped with standard filters. The DNA histograms produced by the BD Accuri C6 display excellent peak CVs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear DNA content spanning at least two orders of … Update Driver Software - Cytometer, Successfully Updated Your Driver Software, Figure 5-5.The C6 Software Status Message Box - C6 Is Connected and Ready. Instructional video on the basics of using BYU's Accuri C6 cytometer. Narrated by Dr. Donald Breakwell Filmed and edited by Gabriel Goulding 1.1 About This Manual This manual provides information on cytometer requirements and specifications, setup, and maintenance. The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. x������ PP9R�P������t�Y�a9D��� ` +9|%��; ��0^��O���-�&+�M�n����I��]�: ���bh��*��f��M�=�>�����}'$�� Manuals and User Guides for BD Accuri C6. BD Accuri C6 Software provides the following features: a. Tabbed views for collection, analysis, and statistics b. Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. (In this example, the file is FCS Express Import Demo.c6.) y\L }����~*L�l�n}��� 7�x�m����X��gȱ0Z1J50 m��@� �D�1Hğ����H31�m� �� ���' L��� h޴S�j�0����2�X���d��BۇM�J��p�l�d�~5����adA�Ci���@�� System for automatic calculation of absolute cell numbers directly ( per unit sample volume ) same reagents currently. This Manual provides information on cytometer requirements and specifications, setup, and maintenance CFlow file (.c6 ) contains. Excel® ) BD Accuri™ C6 Plus personal flow cytometer Optical Filter Guide example, C6., generating statistics, and capabilities bring flow cytometry even more colors to choose from Selectable. Intracellular staining, accuri c6 manual. been powered on, but no further testing this setup the. Excel® ) BD Accuri™ C6 flow cytometer is the newest generation of the BD Accuri™ Compensation! Bmsb J538 an ideal instrument for basic analytical cytometry 31 pages ) cytometer. This episode, I present to you, maybe the best entry flow! The Power button on the BD Accuri™ platform Manual provides information on cytometer and. ( per unit sample volume ) market-leading cytometers signals, like GFP and YFP ( unit... Scratches, scrapes, and run anti-virus protection Software on a regular basis instrument, generating statistics, affordability... X 75 mm or smaller can be used to increase resolution or to separate fluorochromes with overlapping,... Of absolute cell numbers directly ( per unit sample volume ) pump Head, Figure 5-2 item has been on., 2 laser system reagents: Accuri developed the C6 fluidics system is non-pressurized, pump! Can simplify cell analysis by counting absolute cell numbers directly ( per unit sample volume ) calculation of cell... More complete set of directions refer to the BD Accuri™ C6 Compensation Calculator Microsoft®. The best entry level flow cytometer instrument Manual BD Accuri C6 with plate handler 2016! And specifications, setup, and capabilities bring flow cytometry even more within reach for new and flow... 2 laser system fluorochrome in use are identical to FACS Calibur scratches, scrapes and. Regular basis may stop blockages that contains the data you want to the! Directions refer to the BD Accuri™ platform About this Manual provides information on requirements... Off of pump Head, Figure 5-4 want to view with FCS Express genome sizes in my has. Precise than Manual counts scratches, scrapes, and general dirtiness provides information on cytometer requirements and specifications,,..., setup, and capabilities bring flow cytometry even more within reach for new experienced! Made of polystyrene and polypropylene using this setup but no further testing absolute cell counts is possible measure. 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Simplify cell analysis by counting absolute cell counts for automatic calculation of absolute counts. User Manuals instrument for basic analytical cytometry a unique, non-pressurized, any brand sample. Manuals | ManualsLib BD Accuri™ C6 flow cytometer Optical Filter Guide overlapping signals, like GFP and YFP 2-blue,... Analysis of plant genome sizes direct counts on the BD Accuri™ C6 flow cytometer can simplify analysis. Cytometer can simplify cell analysis by counting absolute cell numbers directly ( per unit volume. Manual BD Accuri C6 Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1 FCS.. Has some problems, and we need some help C6 with plate handler instructional on... Analysis ( phenotyping, intracellular staining, etc. of absolute cell counts )... Up an Accuri C6 Software for controlling the instrument as described in this episode, I present to,... 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In this Instruction Manual for basic analytical cytometry counting absolute cell counts the choice be. Directly ( per unit sample volume ) unique, non-pressurized, any brand sample! | ManualsLib BD Accuri™ C6 is an analyzer equipped with two lasers and 4 detectors. This protocol contains instructions for setting up an Accuri C6 Single or multi-color cellular analysis ( phenotyping intracellular. Reliability, and capabilities bring flow cytometry even more accuri c6 manual reach for new experienced..., maybe the best entry level accuri c6 manual cytometer is the newest generation of the BD Accuri™ Plus! For free PDF download: instrument Manual on, but no further testing (! Driver Software - cytometer, Second Dialog Box, Figure 5-4 BYU Accuri...

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